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  1. Singh D, Paduch DA, Schlegel PN, Orwig KE, Mielnik A, Bolyakov A, Wright WW. The production of glial cell line-derived neurotrophic factor by human sertoli cells is substantially reduced in sertoli cell-only testes. 2017. Hum Reprod. 5: 1108-1117. [PUBMED]
  2. Gassei K, Sheng Y, Fayomi A, Mital P, Sukhwani M, Lin CC, Peters KA, Althouse A, Valli H, Orwig KE. DDX4-EGFP transgenic rat model for the study of germline development and spermatogenesis. 2017. Biol Reprod. 3: 707-719. [PUBMED]
  3. Johnson EK, Finlayson C, Rowell EE, Gosiengfiao Y, Pavone ME, Lockart B, Orwig KE, Brannigan RE, Woodruff TK. Fertility Preservation for Pediatric Patients: Current State and Future Possibilities. 2017. J Urol. 17: 30223-9. [PUBMED]
  4. Sosa E, Kim R, Rojas EJ, Hosohama L, Hennebold JD, Orwig KE, Clark AT. An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC89) from embryonic fibroblasts. 2016. Stem Cell Res. 2:444-447. [PUBMED]
  5. Panula S, Reda A, Stukenborg JB, Ramathal C, Sukhwani M, Albalushi H, Edsgärd D, Nakamura M, Söder O, Orwig KE, Yamanaka S, Reijo Pera RA, Hovatta O. Over Expression of NANOS3 and DAZL in Human Embryonic Stem Cells. 2016. PLos One. 11(10):e0165268. [PUBMED]
  6. Song HW, Bettegowda A, Lake BB, Zhao AH, Skarbrevik D, Babajanian E, Sukhwani M, Shum EY, Phan MH, Plank TD, Richardson ME, Ramaiah M, Sridhar V, de Rooij DG, Orwig KE, Zhang K, Wilkinson MF. The Homeobox Transcription Factor RHOX10 Drives Mouse Spermatogonial Stem Cell Establishment. 2016. Cell Reports. 1:149-64. [PUBMED]
  7. Simerly C, Castro C, Hartnett C, Lin CC, Sukhwani M, Orwig K, Schatten G. Post-Testicular Sperm Maturation: Centriole Pairs, Found in Upper Epididymis, are Destroyed Prior to Sperm's Release at Ejaculation. 2016. Sci Rep. 6:31816. [PUBMED]
  8. Gassei K, Orwig KE.  Experimental methods to preserve male fertility and treat male factor infertility. 2016. Fertil Steril. 2:556-566. [PUBMED]
  9. Toocheck C, Clister T, Shupe J, Crum C, Ravindranathan P, Lee TK, Ahn JM, Raj GV, Sukhwani M, Orwig KE, Walker WH. Mouse spermatogenesis requires classical and nonclassical testosterone signaling. 2016. Biol Reprod. 1:11. [PUBMED]
  10. Pech MF, Garbuzov A, Hasegawa K, Sukhwani M, Zhang RJ, Benayoun BA,  Brockman SA, Lin S, Brunet A, Orwig KE, Artandi SE. High telomerase is a hallmark of undifferentiated spermatogonia and is required for maintenance of male germline stem cells. 2015. Genes Dev. 23:2420-2434. [PUBMED]
  11. Ramathal C, Angulo B, Sukhwani M, Cui J, Durruthy-Durruthy J,  Fang F, Schanes P, Turek PJ, Orwig KE, Reijo Pera RA. DDX3Y gene rescue of a Y chromosome AZFa deletion restores germ cell formation and transcriptional programs. 2015. Sci Rep. 5:15041. [PUBMED]
  12. Dominguez AA, Chiang HR, Sukhwani M, Orwig KE, Reijo Pera RA. Human germ cell formation in xenotransplants of induced pluripotent stem cells carrying X chromosome aneuploidies. 2014. Sci Rep. 4:6432. [PUBMED]
  13. Gassei K, Valli H, Orwig KE. Whole-mount immunohistochemistry to study spermatogonial stem cells and spermatogenic lineage development in mice, monkeys, and humans. 2014. Methods Mol Biol. 1210:193-202. [PUBMED]
  14. Medrano JV, Martínez-Arroyo AM, Sukhwani M, Noguera I,  Quiñonero A, Martínez-Jabaloyas JM, Pellicer A, Remohí J, Orwig KE, Simón C. 2014. Germ cell transplantation into mouse testes procedure.  2014. Fertil Steril. 102(4):e11-2. [PUBMED]
  15. Valli H, Sukhwani M, Dovey SL, Peters KA, Donohue J, Castro CA, Chu T, Marshall GR, Orwig KE. Fluorescence- and magnetic-activated cell sorting strategies to isolate and enrich human spermatogonial stem cells. 2014. Fertil Steril. 102(2):566-580. [PUBMED]
  16. Ramathal C, Durruthy Durruthy J, Sukhwani M, Arakaki JE, Turek PJ, Orwig KE, Reijo Pera RA. Fate of iPSCs derived from azoospermic and fertile men following xenotransplantation to murine seminiferous tubules. 2014. Cell Rep. 7(4):1284-97. [PUBMED]
  17. Durruthy Durruthy J, Ramathal C, Sukhwani M, Arakaki JE, Turek PJ, Orwig KE, Reijo Pera RA. Fate of induced pluripotent stem cells following transplantation to murine seminiferous tubules. 2014. Hum Mol Genet 23(12):3071-84. [PUBMED]
  18. Valli H, Phillips BT, Shetty G, Byrne JA, Clark AT, Meistrich ML, Orwig KE. Germline stem cells: toward the regeneration of spermatogenesis. 2014. Fertil Steril 101(1):3-13. [PUBMED]
  19. Zhang Z, Zhang S, Huang X, Orwig KE, Sheng Y. 2013. Rapid Assembly of Customized TALENs into Multiple Delivery Systems. PLoS One  8(11):e80281. [PUBMED]
  20. Shetty G, Uthamanthil RK, Zhou W, Shao SH, Weng CC, Tailor RC, Herman BP, Orwig KE, Meistrich ML. 2013. Hormone suppression with GnRH antagonist promotes spermatogenic recovery from transplanted spermatogonial stem cells in irradiated cynomolgus monkeys. Andrology 1(6):886-98. [PUBMED]
  21. Dovey SL, Valli H, Hermann BP, Sukhwani M, Donohue J, Castro CA, Chu T, Sanfilippo JS, Orwig KE. 2013. Eliminating malignant contamination from therapeutic human spermatogonial stem cells. J Clin Invest 123(4):1833-43. [PUBMED]
  22. Skaznik-Wikiel ME, McGuire MM, Sukhwani M, Donohue J, Chu T, Krivak TC, Rajkovic A, Orwig KE. 2013. Granulocyte colony-stimulating factor with or without stem cell factor extends time to premature ovarian insufficiency in female mice treated with alkylating chemotherapy. Fertil Steril. 99(7):2045-54. [PUBMED]
  23. Gassei K and Orwig KE. 2013. Sall4 expression in gonocytes and spermatogonial clones of postnatal mouse testes. PLoS One. 8(1):e53976. [PUBMED]
  24. Hermann BP, Sukhwani M, Winkler F, Pascarella JN, Peters KA, Sheng Y, Valli H, Rodriguez M, Ezzelarab M, Dargo G, Peterson K, Masterson K, Ramsey C, Ward T, Lienesch M, Volk A, Cooper DK, Thomson AW, Kiss JE, Penedo MC, Schatten GP, Mitalipov S, Orwig KE. 2012. Spermatogonial stem cell transplantation into rhesus testes regenerates spermatogenesis producing functional sperm. Cell Stem Cell 11(5):715-26. [PUBMED]
  25. Easley CA 4th, Phillips BT, McGuire MM, Barringer JM, Valli H, Hermann BP, Simerly CR, Rajkovic A, Miki T, Orwig KE, Schatten GP. Direct differentiation of human pluripotent stem cells into haploid spermatogenic cells. 2012. Cell Rep. 2(3):440-6. [PUBMED]
  26. Suzuki H, Ahn HW, Chu T, Bowden W, Gassei K, Orwig K, Rajkovic A. 2012. SOHLH1 and SOHLH2 coordinate spermatogonial differentiation. Dev Biol. 361(2):301-12. [PUBMED]
  27. Clark AT, Phillips BT, Orwig KE. 2011. Fruitful progress to fertility: male fertility in the test tube. Nat Med. 17(12):1564-5. [PUBMED]
  28. Hermann BP, Sukhwani M, Salati J, Sheng Y, Chu T, Orwig KE. 2011. Separating spermatogonia from cancer cells in contaminated prepubertal primate testis cell suspensions. Hum Reprod. 26(12):3222-31. [PUBMED]
  29. Hermann BP, Phillips BT, Orwig KE. 2011. The elusive spermatogonial stem cell marker? Biol Reprod. Aug;85(2):221-3. [PUBMED]
  30. Honaramooz A, Schlatt S, Orwig K, Kim NH. Recent advances in reproductive technologies. 2011. Vet Med Int. 915031. Epub 2011 Jun 22 .[PUBMED]
  31. Simerly C, McFarland D, Castro C, Lin CC, Redinger C, Jacoby E, Mich-Basso J, Orwig K, Mills P, Ahrens E,  Navara C, Schatten G. 2011. Interspecies chimera between primate embryonic stem cells and mouse embryos: monkey ESCs engraft into mouse embryos, but not post-implantation fetuses. Stem Cell Res. 7(1):28-40. [PUBMED]
  32. Phillips BT, Gassei K , Orwig KE. 2010. Spermatogonial stem cell regulation and spermatogenesis. Phil Trans R Soc B ; 365, 1663-1678. [PUBMED]
  33. Ben-Yehudah A, Easley 4th CA, Hermann BP, Castro C, Simerly C, Orwig KE, Mitalipov S, Schatten G. 2010. Systems biology discoveries using non-human primate pluripotent stem and germ cells: novel gene and genomic imprinting interactions as well as unique expression patterns.Stem Cell Res Ther. 1(3):24. [PUBMED]
  34. Hermann BP, Sukhwani M, Hansel MC, Orwig KE. 2010. Spermatogonial stem cells in higher primates: are there differences from those in rodents? Reproduction 139: 479–493; DOI: 10.1530/REP-09-0255 [ABSTRACT]
  35. Sheng Y, Lin CC, Yue J, Sukhwani M, Shuttleworth JJ, Chu T, Orwig KE. Generation and characterization of a Tet-On (rtTA-M2) transgenic rat. 2010. BMC Developmental Biology, 10:17. [ABSTRACT]
  36. Hermann BP, Sukhwani M, Simorangkir DR, Chu T, Plant TM, Orwig KE. 2009. Molecular Dissection of the Male Germ Cell Lineage Identifies Putative Spermatoganial Stem Cells in Rhesus Macaques. Hum Reprod. 24(7): 1704-16. [PUBMED]
  37. Escamilla-Hernandez R, Little-Ihrig L , Orwig KE , Yue J, Chandran U, Zeleznik AJ. 2008. Constitutively Active Protein Kinase A Qualitatively Mimics the Effects of Follicle-Stimulating Hormone on Granulosa Cell Differentiation. Mol Endocrinol. 22(8):1842-52. [PUBMED]
  38. Orwig KE, Ryu BY, Master S, Phillips BT, Mack M, Avarbock MR, Brinster RL. 2008. Genes Involved in Post-transcriptional Regulation are Over Represented in Stem/progenitor Spermatogonia of Cryptorchid Mouse Testes. Stem Cells. 26(4):927-38. [PUBMED]
  39. Yue J, Sheng Y, Orwig KE. 2008. Identification of Novel Homologous microRNA Genes in the Rhesus Macaque Genome. BMC Genomics. 9(1): 8. [PUBMED]
  40. Hermann BP, Sukhwani M, Lin CC, Sheng Y, Tomko J, Rodriguez M, Shuttleworth JJ, McFarland D, Hobbs RM, Pandolfi PP, Schatten GP, Orwig KE. 2007. Characterization, Cryopreservation and Ablation of Spermatogonial Stem Cells In Adult Rhesus Macaques. Stem Cells 25(9):2330-2338. [PUBMED]
  41. Navara CS, Mich-Basso JD, Redinger CJ, Ben-Yehudah A, Jacoby E, Kovkarova-Naumovski E, Sukhwani M, Orwig K, Kaminski N, Castro CA, Simerly CR, Schatten G. 2007. Pedigreed Primate Embryonic Stem Cells, Express Homogeneous Familial Gene Profiles. Stem Cells. 25(11):2695-704. [PUBMED]
  42. Ryu BY, Orwig KE,  Oatley JM, Lin CC, Chang LJ, Avarbock MR, Brinster RL. 2007. Efficient Generation of Transgenic Rats Through the Male Germline using Lentiviral Transduction and Transplantation of Spermatogonial Stem Cells. J Androl. 28(2):353-60. [PUBMED]
  43. Ryu BY, Orwig KE, Oatley JM, Avarbock MR, Brinster RL. 2006. Effects of Aging and Niche Microenvironment on Spermatogonial Stem Cell Self-Renewal.  Stem Cells. 24(6):1505-1511. [PUBMED]
  44. Buageaw A, Sukhwani M, Ben-Yehudah A, Ehmcke J, Rawe VY,  Pholpramool C, Orwig KE, Schlatt S. 2005. GDNF Family Receptor alpha1 Phenotype of Spermatogonial Stem Cells in Immature Mouse Testes. Biol. Reprod. 73(5):1011-1016. [PUBMED]
  45. Orwig KE, Schlatt S. 2005. Cryopreservation and transplantation of spermatogonia and testicular tissue for preservation of male fertility.J Natl Cancer Inst Monogr. 34:51-56. [PUBMED]
  46. Ryu BY, Orwig KE, Kubota H, Avarbock MR, Brinster RL. 2004. Phenotypic and functional characteristics of spermatogonial stem cells in rats. Dev. Biol. 274:158-170. [PUBMED]
  47. Costoya JA, Hobbs RM, Barna M, Cattoretti G, Manova K, Sukhwani M, Orwig KE, Wolgemuth DJ, Pandolfi PP. 2004. Essential role of Plzf in maintenance of spermatogonial stem cells. Nature Genetics. 36: 653-659. [PUBMED]
  48. B.Y. Ryu, K.E. Orwig, M.R. Avarbock, R.L. Brinster. 2003. Stem cell and niche development in the postnatal rat testis. Dev. Biol. 263:253-263. [PUBMED]
  49. T. Shinohara, K.E. Orwig, M.R. Avarbock, R.L. Brinster. 2003. Restoration of spermatogenesis in infertile mice by Sertoli cell transplantation. Biol. Reprod. 68:1064-1071. [PUBMED]
  50. C.J. Brinster, B.-Y. Ryu, L. Karagenc, R.L. Brinster, K.E. Orwig. 2003. Restoration of fertility by germ cell transplantation requires effective recipient preparation. Biol. Reprod. 69:412-420. [PUBMED]
  51. K.E. Orwig, B.-Y. Ryu, M.R. Avarbock, R.L. Brinster. 2002. Male germ-line stem cell potential is predicted by morphology of cells in neonatal rat testes. Proc. Nat. Acad. Sci. USA. 99:11706-11711. [PUBMED]
  52. K.E. Orwig, M.R. Avarbock, R.L. Brinster. 2002. Retrovirus-mediated modification of male germline stem cells in rats. Biol. Reprod. 67:874-879. [PUBMED]
  53. T. Shinohara, K.E. Orwig, M.R. Avarbock, R.L. Brinster. 2002. Germ line stem cell competition in postnatal mouse testes. Biol. Reprod. 66:1491-1497. [PUBMED]
  54. K.E. Orwig, T. Shinohara, M.R. Avarbock, R.L. Brinster. 2002. Functional analysis of stem cells in the adult rat testis. Biol. Reprod. 66:944-949. [PUBMED]
  55. M. Nagano, C.J. Brinster, K.E. Orwig, B.-Y. Ryu, M.R. Avarbock, R.L. Brinster. 2001. Transgenic mice produced by retroviral transduction of male germ-line stem cells. Proc. Nat. Acad. Sci. USA. 98:13090-13095. [PUBMED]
  56. T. Shinohara, K.E. Orwig, M.R. Avarbock, R.L. Brinster. 2001. Remodeling of the postnatal mouse testis is accompanied by dramatic changes in stem cell number and niche accessibility. Proc. Nat. Acad. Sci. USA. 98:6186-6191. [PUBMED]
  57. T. Shinohara, K.E. Orwig, M.R. Avarbock, R.L. Brinster. 2000. Spermatogonial stem cell enrichment by multiparameter selection of mouse testis cells. Proc. Nat. Acad. Sci. USA. 97:8346-8351. [PUBMED]
  58. S. Filley, S. Supansic, U. Salli, K. Orwig, F. Stormshak. 2000. Myristoylated alanine-rich c kinase substrate protein and mRNA in bovine corpus luteum during the estrous cycle. Endocrine 12:289-294. [PUBMED]
  59. K.E. Orwig and M.J. Soares. 1999. Transcriptional activation of the decidual/trophoblast prolactin-related protein gene. Endocrinology. 140:4032-4039. [PUBMED]
  60. G.D. Dai, B.M. Chapman, B. Liu, K.E. Orwig, D. Wang, R.A. White, B. Preuett, M.M. Soares. 1998. A new member of the mouse prolactin (PRL)-like protein-C subfamily, PRL-like protein-Ca: Structure and Expression. Endocrinology 139:5157-5163. [PUBMED]
  61. K.E. Orwig, M.W. Wolfe, C.B. Cohick, G. Dai, T.J. Peters, M.J. Soares. 1998. Trophoblast-specific regulation of endocrine-related genes. Trophoblast Research 12:65-85.
  62. H. Müller, K.E. Orwig, M.J. Soares. 1998. Identification of two new members of the mouse prolactin gene family. Biochim. Biophys. Acta 1396:251-258. [PUBMED]
  63. M.J. Soares, H. Müller, K.E. Orwig, T.J. Peters, G. Dai. 1998. The Uteroplacental prolactin family and pregnancy. Biol. Reprod. 58:273-284. [PUBMED]
  64. H. Müller, R. Ishimura, K.E. Orwig, B. Liu, M.J. Soares. 1998. Homologues for prolactin-like protein-A and B are present in the mouse. Biol. Reprod. 58:45-51. [PUBMED]
  65. K.E. Orwig, R. Ishimura, H. Müller, B. Liu and M.J. Soares. 1997. Identification and characterization of a mouse homolog for decidual/trophoblast prolactin-related protein. Endocrinology 138:5511-5517. [PUBMED]
  66. K.E. Orwig, C.A. Rasmussen and M.J. Soares. 1997. Decidual Signals in the establishment of pregnancy: the prolactin family. Trophoblast Research 10:329-343.
  67. K.E. Orwig, G. Dai, C.A. Rasmussen and M.J. Soares. 1997. Decidual/trophoblast prolactin-related protein: characterization of gene structure and cell-specific expression. Endocrinology 138:2491-2500. [PUBMED]
  68. C.A. Rasmussen, K.E. Orwig, S. Vellucci and M.J. Soares. 1997. Dual expression of prolactin-related protein in decidua and trophoblast tissues during pregnancy. Biol. Reprod. 56:647-654. [PUBMED]
  69. C.A. Rasmussen, K. Hashizume, K.E. Orwig, L. Xu and M.J. Soares. 1996. Decidual prolactin-related protein: heterologous expression and characterization. Endocrinology 137:5558-5566. [PUBMED]
  70. M.J. Soares, B.M. Chapman, C.B. Cohick, C.A. Rasmussen, G. Dai, T. Kamei and K.E. Orwig. 1996. Differentiation of trophoblast endocrine cells. Placenta 17:277-289. [PUBMED]
  71. F. Stormshak, K.E. Orwig and J.E. Bertrand. 1995. Dynamics of molecular mechanisms underlying ovarian oxytocin secretion. Journal of Reproduction and Fertility. 49:379-390. [PUBMED]
  72. K.E. Orwig, J.E. Bertrand, B.-R.Ou, N.E. Forsberg and F. Stormshak. 1994. Immunochemical characterization and cellular distribution of protein kinase C isozymes in the bovine corpus luteum. Comparative Biochemistry and Physiology. 108B:53-57. [PUBMED]
  73. K.E. Orwig, J.E. Bertrand, B.-R.Ou, N.E. Forsberg and F.Stormshak. 1994. Involvement of protein kinase C, calpains and calpastatin in prostaglandin F2*-induced oxytocin secretion from the bovine corpus luteum. Endocrinology. 134:78-83. [PUBMED]
  74. K.E. Orwig, S. Leers-Sucheta, M.F. Moghaddam, Z.D. Jiang, W.R. Gerwick and F. Stormshak. 1992. Unique metabolites of eicosapentaenoic acid interfere with corpus luteum function in the ewe. Prostaglandins. 44:519-530. [PUBMED]

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