Publications

  1. Nahata L, Halpern JA, Bendle K, Frias O, Orwig K, Reinecke J, Smith JF, Woodard T, Levine J. The Case for Pre-Treatment Sperm Banking as Standard of Care for All Post-Pubertal Males with Cancer. J Adolesc Young Adult Oncol. 2024 Sep 30. doi: 10.1089/jayao.2024.0100. [PUBMED]
  2. Ausavarungnirun C, Orwig KE. REPRODUCTIVE HEALTH IN TRANS AND GENDER-DIVERSE PATIENTS: Gonadal tissue cryopreservation in transgender and gender-diverse people. Reproduction. 2024 Nov 25;168(6):e240253. doi: 10.1530/REP-24-0253. [PUBMED]
  3. Safrai M, Orwig KE. Utilizing artificial intelligence in academic writing: an in-depth evaluation of a scientific review on fertility preservation written by ChatGPT-4. J Assist Reprod Genet. 2024 Jul;41(7):1871-1880. doi: 10.1007/s10815-024-03089-7. [PUBMED]
  4. Duffin K, Neuhaus N, Andersen CY, Barraud-Lange V, Braye A, Eguizabal C, Feraille A, Ginsberg JP, Gook D, Goossens E, Jahnukainen K, Jayasinghe Y, Keros V, Kliesch S, Lane S, Mulder CL, Orwig KE, van Pelt AMM, Poirot C, Rimmer MP, Rives N, Sadri-Ardekani H, Safrai M, Schlatt S, Stukenborg JB, van de Wetering MD, Wyns C, Mitchell RT. A 20-year overview of fertility preservation in boys: new insights gained through a comprehensive international survey. Hum Reprod.
    2024 Feb 16;2024(2):hoae010. doi: 10.1093/hropen/hoae010. [PUBMED]
  5. Buckenmeyer MJ, Sukhwani M, Iftikhar A, Nolfi AL, Xian Z, Dadi S, Case ZW, Steimer SR, D’Amore A, Orwig KE, Brown BN. A bioengineered in situ ovary (ISO) supports follicle engraftment and live-births post-chemotherapy. J Tissue Eng. 2023 Nov 17:14:20417314231197282. doi: 10.1177/20417314231197282. [PUBMED]
  6. Harancher MR, Sukhwani M, Castro CA, Hsieh MH, Orwig KE. Generation of two isogenic sickle cell disease induced pluripotent stem cell lines from testicular fibroblasts. Stem Cell Res. 2023 Dec:73:103257. doi: 10.1016/j.scr.2023.103257. [PUBMED]
  7. Nam CS, Campbell KJ, Acquati C, Bole R, Adler A, Collins DJ, Collins E, Samplaski M, Anderson-Bialis J, Andino JJ, Asafu-Adjei D, Gaskins AJ, Bortoletto P, Vij SC, Orwig KE, Lundy SD. Deafening Silence of Male Infertility. Urology. 2023 Dec:182:111-124. doi: 10.1016/j.urology.2023.09.018. [PUBMED]
  8. Younis N, Caldeira-Brant AL, Chu T, Abdalla S, Orwig KE. Human immature testicular tissue organ culture: a step towards fertility preservation and restoration. Front Endocrinol (Lausanne). 2023 Aug 28:14:1242263. doi: 10.3389/fendo.2023.1242263. [PUBMED]
  9. Moritz L, Schon SB, Rabbani M, Sheng Y, Agrawal R, Glass-Klaiber J, Sultan C, Camarillo JM, Clements J, Baldwin MR, Diehl AG, Boyle AP, O’Brien PJ, Ragunathan K, Hu YC, Kelleher NL, Nandakumar J, Li JZ, Orwig KE, Redding S, Hammoud SS. Sperm chromatin structure and reproductive fitness are altered by substitution of a single amino acid in mouse protamine 1. Nat Struct Mol Biol. 2023 Aug;30(8):1077-1091. doi: 10.1038/s41594-023-01033-4. [PUBMED]
  10. Ding X, Singh P, Schimenti K, Tran TN, Fragoza R, Hardy J, Orwig KE, Olszewska M, Kurpisz MK, Yatsenko AN, Conrad DF, Yu H, Schimenti JC. In vivo versus in silico assessment of potentially pathogenic missense variants in human reproductive genes. Proc Natl Acad Sci U S A. 2023 Jul 25;120(30):e2219925120. doi: 10.1073/pnas.2219925120. [PUBMED]
  11. Zhang X, Liu Y, Sosa F, Gunewardena S, Crawford PA, Zielen AC, Orwig KE, Wang N. Transcriptional metabolic reprogramming implements meiotic fate decision in mouse testicular germ cells. Cell Rep. 2023 Jul 25;42(7):112749. doi: 10.1016/j.celrep.2023.112749. [PUBMED]
  12. Rajachandran S, Zhang X, Cao Q, Caldeira-Brant AL, Zhang X, Song Y, Evans M, Bukulmez O, Grow EJ, Nagano M, Orwig KE, Chen H. Dissecting the spermatogonial stem cell niche using spatial transcriptomics. Cell Rep. 2023 Jul 25;42(7):112737. doi: 10.1016/j.celrep.2023.112737. [PUBMED]
  13. Munyoki SK, Orwig KE. Perspectives: Methods for Evaluating Primate Spermatogonial Stem Cells. Methods Mol Biol. 2023;2656:341-364. doi: 10.1007/978-1-0716-3139-3_18. [PUBMED]
  14. Qureshi S, Hardy JJ, Pombar C, Berman AJ, Malcher A, Gingrich T, Hvasta R, Kuong J,  Munyoki S, Hwang K, Orwig KE, Ahmed J,  Olszewska M, Kurpisz M, Conrad DF, Khan MJ,  Yatsenko AN. Genomic study of TEX15 variants: prevalence and allelic heterogeneity in men with spermatogenic failure. Front Genet. 2023; 14: 1134849. Published online 2023 May 10. doi: 10.3389/fgene.2023.1134849. [PUBMED]
  15. Yap YT, Li W, Huang Q, Zhou Q, Zhang D, Sheng Y, Mladenovic-Lucas L, Yee S-P, Orwig KE,  Granneman JG, Williams DC Jr, Hess RA,  Toure A, Zhang Z. DNALI1 interacts with the MEIG1/PACRG complex within the manchette and is required for proper sperm flagellum assembly in mice. eLife. 2023; 12: e79620. Published online 2023 Apr 21. doi: 10.7554/eLife.79620. [PUBMED]
  16. Nie X, Munyoki SK, Sukhwani M, Schmid N,  Missel A, Emery BR, DonorConnect,  Stukenborg J-B,  Mayerhofer A, Orwig KE, Aston KI,  Hotaling JM, Cairns BR, Guo J. Single-Cell Analysis of Human Testis Aging and Correlation with Elevated Body Mass Index. Dev Cell. 2022 May 9; 57(9): 1160–1176.e5. [PUBMED]
  17. Pelzman D, Dederer G, Joolharzadeh P, Morrill C, Orwig K, Pulaski H, Hwang K. Effect of Distance From Fertility Center on Utilization of Fertility Preservation Referral in Men. JCO Oncol Pract 2023;OP2200789. doi: 10.1200/OP.22.00789. [PUBMED]
  18. Costa GMJ, Lacerda SMSN, Figueiredo AFA, Wnuk NT, Brener MRG, Andrade LM, Campolina-Silva GH, Kauffmann-Zeh A, Pacifico LGG, Versiani AF, Antunes MM, Souza FR, Cassali GD, Caldeira-Brant AL, Chiarini-Garcia H, de Souza FG, Costa VV, da Fonseca FG, Nogueira ML, Campos GRF, Kangussu LM, Martins EMN, Antonio LM, Bittar C, Rahal P, Aguiar RS, Mendes BP, Procópio MS, Furtado TP, Guimaraes YL, Menezes GB, Martinez-Marchal A, Orwig KE, Brieño-Enríquez M, Furtado MH. High SARS-CoV-2 tropism and activation of immune cells in the testes of non-vaccinated deceased COVID-19 patients. BMC Biol 2023;21(1):36. doi: 10.1186/s12915-022-01497-8. [PUBMED]
  19. Siebert AL, Gomez-Lobo V, Johnson EK, Nahata L, Orwig KE, Pyle LC, Witchel SF, Finlayson C, Laronda MM. Differences in gonadal tissue cryopreservation practices for differences of sex development across regions in the United States. Front Endocrinol (Lausanne). 2023;13:990359. doi: 10.3389/fendo.2022.990359. eCollection 2022. [PUBMED]
  20. Voigt AL, Dardari R, Lara NLM, He T, Steele H, Dufour A, Orwig KE, Dobrinski I. Multiomics approach to profiling Sertoli cell maturation during development of the spermatogonial stem cell niche. Mol Hum Reprod. 2023;29(3):gaad004. doi: 10.1093/molehr/gaad004. [PUBMED]
  21. Ataman LM, Laronda MM, Gowett M, Trotter K, Anvari H, Fei F, Ingram A, Minette M, Suebthawinkul C, Taghvaei Z, Torres-Vélez M, Velez K, Adiga SK, Anazodo A, Appiah L, Bourlon MT, Daniels N, Dolmans MM, Finlayson C, Gilchrist RB, Gomez-Lobo V, Greenblatt E, Halpern JA, Hutt K, Johnson EK, Kawamura K, Khrouf M, Kimelman D, Kristensen S, Mitchell RT, Moravek MB, Nahata L, Orwig KE, Pavone ME, Pépin D, Pesce R, Quinn GP, Rosen MP, Rowell E, Smith K, Venter C, Whiteside S, Xiao S, Zelinski M, Goldman KN, Woodruff TK, Duncan FE. A synopsis of global frontiers in fertility preservation. J Assist Reprod Genet 2022;39(8):1693-1712. doi: 10.1007/s10815-022-02570-5. [PUBMED]
  22. Voigt AL, Dardari R, Su L, Lara NLM, Sinha S, Jaffer A, Munyoki SK, Alpaugh W, Dufour A, Biernaskie J, Orwig KE, Dobrinski I. Metabolic transitions define spermatogonial stem cell maturation. Hum Reprod 2022;37(9):2095-2112. doi: 10.1093/humrep/deac157. [PUBMED]
  23. Nie X, Munyoki SK, Sukhwani M, Schmid N, Missel A, Emery BR, DonorConnect, Stukenborg JB, Mayerhofer A, Orwig KE, Aston KI, Hotaling JM, Cairns BR, Guo J. Single-cell analysis of human testis aging and correlation with elevated body mass index. Dev Cell 2022, 9;57(9):1160-1176.e5. doi: 10.1016/j.devcel.2022.04.004. [PUBMED]
  24. Tran KTD, Valli-Pulaski H, Colvin A, Orwig KE. Male fertility preservation and restoration strategies for patients undergoing gonadotoxic therapies. Biol Reprod
     2022;107(2):382-405. doi: 10.1093/biolre/ioac072. [PUBMED]
  25. Tran KTD, Sheng Y, Doungkamchan C, Castro CA, Orwig KE. Induced pluripotent stem cell line from a mouse model of human azoospermia with a frameshift mutation tex11_1260ins(tt). Stem Cell Res 2022;60:102728. [PUBMED]
  26. Teekakirikul P, Zhu W, Xu X, Young CB, Tan T, Smith AM, Wang C, Peterson KA, Gabriel GC, Ho S, Sheng Y, Moreau de Bellaing A, Sonnenberg DA, Lin J-h, Fotiou E, Tenin G, Wang MX, Wu YL, Feinstein T, Devine W, Gou H, Bais AS, Glennon BJ, Zahid M, Wong TC, Ahmad F, Rynkiewicz MJ, Lehman WJ, Keavney B, Alastalo T-P, Freckmann M-L, Orwig K, Murray S, Ware SM, Zhao H, Feingold B, Lo CW. Genetic resiliency associated with dominant lethal tpm1 mutation causing atrial septal defect with high heritability. Cell Reports Medicine 2022;3:100501. [PUBMED]
  27. Khampang S, Cho IK, Punyawai K, Gill B, Langmo JN, Nath S, Greeson KW, Symosko KM, Fowler KL, Tian S, Statz JP, Steves AN, Parnpai R, White MA, Hennebold JD, Orwig KE, Simerly CR, Schatten G, Easley CAIV. Blastocyst development after fertilization with in vitro spermatids derived from nonhuman primate embryonic stem cells. F&S Science 2021;2:365-375. [PUBMED]
  28. Shetty G, Mitchell JM, Lam TNA, Phan TT, Zhang J, Tailor RC, Peters KA, Penedo MC, Hanna CB, Clark AT, Orwig KE, Meistrich ML. Postpubertal spermatogonial stem cell transplantation restores functional sperm production in rhesus monkeys irradiated before and after puberty. Andrology 2021. [PUBMED]
  29. Shen Y-C, Shami AN, Moritz L, Larose H, Manske GL, Ma Q, Zheng X, Sukhwani M, Czerwinski M, Sultan C, Chen H, Gurczynski SJ, Spence JR, Orwig KE, Tallquist M, Li JZ, Hammoud SS. Tcf21+ mesenchymal cells contribute to testis somatic cell development, homeostasis, and regeneration in mice. Nat Commun 2021; 12:3876. [PUBMED]
  30. Hardy JJ, Wyrwoll MJ, Mcfadden W, Malcher A, Rotte N, Pollock NC, Munyoki S, Veroli MV, Houston BJ, Xavier MJ, Kasak L, Punab M, Laan M, Kliesch S, Schlegel P, Jaffe T, Hwang K, Vukina J, Brieño-Enríquez MA, Orwig K, Yanowitz J, Buszczak M, Veltman JA, Oud M, Nagirnaja L, Olszewska M, O’Bryan MK, Conrad DF, Kurpisz M, Tüttelmann F, Yatsenko AN. Variants in GCNA, X-linked germ-cell genome integrity gene, identified in men with primary spermatogenic failure. GEMINI Consortium. Hum Genet. 2021 May 7. doi: 10.1007/s00439-021-02287-y. [PUBMED]
  31. Voigt AL, Kondro DA, Powell D, Valli-Pulaski H, Ungrin M, Stukenborg JB, Klein C, Lewis IA, Orwig KE, Dobrinski I. Unique metabolic phenotype and its transition during maturation of juvenile male germ cells. FASEB J. 2021 May;35(5):e21513. doi: 10.1096/fj.202002799R. [PUBMED]
  32. Hinkle K, Orwig KE, Valli-Pulaski H, Taylor S, van Leeuwen K, Carpentieri D, Walsh A. Cryopreservation of Ovarian Tissue for Pediatric Fertility. Biopreserv Biobank. 2021 Apr 12. doi: 10.1089/bio.2020.0124. [PUBMED]
  33. Parker, N, Laychur A, Sukhwani M, Orwig KE, Oatley JM, Zhang C, Rutaganaria FU, Shokat K, Wright WW. Spermatogonial stem cell numbers are reduced by transient inhibition of GDNF signaling but restored by self-renewing replication when signaling resumes. Stem Cell Reports. 2021 March. 16(3): 597–609. doi: 10.1016/j.stemcr.2021.01.015. [PUBMED]
  34. Practice Committee of Oncofertility Consortium. Installing oncofertility programs for common cancers in optimum resource settings (Repro-Can OPEN study part II): a committee opinion. J Assist Reprod Genet. 2021 Jan 16. doi: 10.1007/s10815-020-02012-0. [PUBMED]
  35. Woodruff TK, Ataman-Millhouse L, Acharya KS, Almeida-Santos T, Anazodo A, Anderson RA, Appiah L, Bader J, Becktell K, Brannigan RE et al. A view from the past into our collective future: the oncofertility consortium vision statement. J Assist Reprod Genet. 2021 Jan 6:1-13.  doi: 10.1007/s10815-020-01983-4. [PUBMED]
  36. Shetty G, Mitchell JM, Lam TNA, Phan TT, Zhang J, Tailor RC, Peters KA, Penedo MC, Hanna CB, Clark AT, Orwig KE, Meistrich ML. Restoration of Functional Sperm Production in Irradiated Pubertal Rhesus Monkeys by Spermatogonial Stem Cell Transplantation. Andrology 2020; doi: 10.1111/andr.12807. [PUBMED]
  37. Yersal N, Köse S, Horzum U, Özkavukcu S, Orwig KE, Korkusuz P. Leptin promotes proliferation of neonatal mouse stem/progenitor spermatogonia. J Assist Reprod Genet. 2020 Aug 25;. doi: 10.1007/s10815-020-01929-w. [PUBMED]
  38. Tan K, Song HW, Thompson M, Munyoki SK, Sukhwani M, Hsieh TC, Orwig KE, Wilkinson MF. Transcriptome profiling reveals signaling conditions dictating human spermatogonia fate in vitro. PNAS. 2020 Jul 13;202000362. doi: 10.1073/pnas.2000362117. [PUBMED]
  39. Goossens E, Jahnukainen K, Mitchell RT, van Pelt A, Pennings G, Rives N, Poels J, Wyns C, Lane S, Rodriguez-Wallberg KA, Rives A, Valli-Pulaski H, Steimer S, Kliesch S, Braye A, Andres MM, Medrano J, Ramos L, Kristensen SG, Andersen CY, Bjarnason R, Orwig KE, Neuhaus N, Stukenborg JB. Fertility Preservation in Boys: Recent Developments and New Insights. Hum Reprod Open. 2020. doi: 10.1093/hropen/hoaa016. eCollection 2020. [PUBMED]
  40. Meacham LR, Burns K, Orwig KE, Levine J. Standardizing risk assessment for treatment-related gonadal insufficiency and infertility in childhood adolescent and young adult cancer: The Pediatric Initiative Network risk stratification system. J Adolesc Young Adult Oncol. 2020 doi: 10.1089/jayao.2020.0012. [PUBMED]
  41. Nahata L, Woodruff TK, Quinn GP, Meacham LR, Chen D, Appiah LC, Finlayson C, Orwig KE, Laronda MM, Rowell EE, Anazodo A, Frias O, Rios JS, Whiteside S, Gomez-Lobo V, Dwiggins M, Childress KJ, Hoefgen HR, Levine JM, Jayasinghe Y, Moravek M. Ovarian tissue cryopreservation as standard of care: what does this mean for pediatric populations?  J Assist Reprod Genet. 2020; doi: 10.1007/s10815-020-01794-7. [PUBMED]
  42. Shami AN, Zheng X, Munyoki SK, Ma Q, Manske GL, Green GD, Sukhwani M, Orwig KE, Li JL*, Hammoud SS. Single-cell RNA sequencing of human, macaque, and mouse testes uncovers conserved and divergent features of mammalian spermatogenesis. Dev Cell 2020, 54(4):529-547.e12. doi: 10.1016/j.devcel.2020.05.010. [PUBMED]
  43. Shetty G, Mitchell JM, Meyer JM, Wu Z, Lam TNA, Phan TT, Zhang J, Hill L, Tailor RC, Peters KA, Penedo MC, Hanna C, Orwig KE, Meistrich ML. Restoration of Functional Sperm Production in Irradiated Pubertal Rhesus Monkeys by Spermatogonial Stem Cell Transplantation. .Andrology 2020; doi: 10.1111/andr.12807. [PUBMED]
  44. Morimoto H, Kanatsu-Shinohara M, Orwig KE, Shinohara T. Expression and functional analyses of EPHA2 in mouse spermatogonial stem cells. Biol Reprod. 2019 Aug 12. pii: ioz156. doi: 10.1093/biolre/ioz156. [PUBMED]
  45. Barnard EP, Dhar CP, Rothenberg SS, Menke MN, Witchel SF, Montano GT, Orwig KE, Valli-Pulaski H. Fertility Preservation Outcomes in Adolescent and Young Adult Feminizing Transgender Patients. Pediatrics. 2019 Sep;144(3). pii: e20183943. doi: 10.1542/peds.2018-3943. [PUBMED]
  46. Valli-Pulaski H, Peters KA, Gassei K, Steimer SR, Sukhwani M, Hermann BP, Dwomor L, David S, Fayomi AP, Munyoki SK, Chu T, Chaudhry R, Cannon GM, Fox PJ, Jaffe TM, Sanfilippo JS, Menke MN, Lunenfeld E, Abofoul-Azab M, Sender LS, Messina J, Klimpel LM, Gosiengfiao Y, Rowell EE, Hsieh MH, Granberg CF, Reddy PP, Sandlow JI, Huleihel M, Orwig KE. Testicular tissue cryopreservation: 8 years of experience from a coordinated network of academic centers. Hum Reprod. 2019; May 21; doi: 10.1093/humrep/dez043. [PUBMED]
  47. Sakib S, Uchida A, Valenzuela-Leon P, Yu Y, Valli-Pulaski H, Orwig K, Ungrin M, Dobrinski I. Formation of organotypic testicular organoids in microwell culture. Biol Reprod. 2019; March 29; doi: 10.1093/biolre/ioz053. [PUBMED]
  48. Fayomi A, Peters K, Sukhwani M, Valli-Pulaski H, Shetty G, Meistrich M, Houser L, Robertson N, Roberts V, Ramsey C, Hanna C, Hennebold J, Dobrinski I, Orwig KE. Autologous Grafting of Cryopreserved Prepubertal Rhesus Testis Produces Sperm and Offspring. Science. 2019; March 22; 363(6433): 1314-1319. doi: 10.1126/science.aav2914. [PUBMED], [PDF]
  49. Corkum KS, Lautz TB, Johnson EK, Reimann MB, Walz AL, Lockart BA, Brannigan RE, ValliPulaski H, Orwig KE, Rowell EE. Testicular wedge biopsy for fertility preservation in children at significant risk for azoospermia after gonadotoxic therapy. J Pediatr Surg. 2019; Feb 21. pii: S0022-3468(19)30107-1. doi: 10.1016/j.jpedsurg.2019.01.055. [PUBMED]
  50. Sosa E, Chen D, Rojas EJ, Hennebold JD, Peters KA, Wu Z, Lam TN, Mitchell JM, Sukhwani M, Tailor RC, Meistrich ML, Orwig KE, Shetty G, Clark AT. Differentiation of primate primordial germ cell-like cells following transplantation into the adult gonadal niche. Nat Commun. 2018;9(1):5339. doi: 10.1038/s41467-018-07740-7. [PUBMED]
  51. Smith BM, Duncan FE, Ataman L, Smith K, Quinn GP, Chang RJ, Finlayson C, Orwig K, Valli-Pulaski H, Moravek MB, Zelinski MB, Irene Su H, Vitek W, Smith JF, Jeruss JS, Gracia C, Coutifaris C, Shah D, Nahata L, Gomez-Lobo V, Appiah LC, Brannigan RE, Gillis V, Gradishar W, Javed A, Rhoton-Vlasak AS, Kondapalli LA, Neuber E, Ginsberg JP, Muller CH, Hirshfeld-Cytron J, Kutteh WH, Lindheim SR, Cherven B, Meacham LR, Rao P, Torno L, Sender LS, Vadaparampil ST, Skiles JL, Schafer-Kalkhoff T, Frias OJ, Byrne J, Westphal LM, Schust DJ, Klosky JL, McCracken KA, Ting A, Khan Z, Granberg C, Lockart B, Scoccia B, Laronda MM, Mersereau JE, Marsh C, Pavone ME, Woodruff TK. The National Physicians Cooperatve: transforming fertility management in the cancer setting and beyond. Future Oncol. 2018; doi: 10.2217/fon-2018-0278. [PUBMED]
  52. Shetty G, Wu Z, Lam TNA, Phan TT, Orwig KE, Meistrich ML. Effect of hormone modulations on donor-derived spermatogenesis or colonization after syngeneic and xenotransplantation in mice. Andrology 2018; doi: 10.1111/andr.12571. [PUBMED]
  53. Shetty G, Mitchell JM, Lam TNA, Wu Z, Zhang J, Hill L, Tailor RC, Peters KA, Penedo MC, Orwig KE, Meistrich ML. Donor spermatogenesis in de novo formed seminiferous tubules from transplanted testicular cells in rhesus monkey testis. Hum Reprod. 2018; 33(12):2249-2255. [PUBMED]
  54. Murdock MH, David S, Swinehart IT, Reing JE, Tran K, Gassei K, Orwig KE and Badylak SF. Human testis extracellular matrix enhances human spermatogonial stem cell survival in vitro. Tissue Engineering Part A. 2018, doi: 10.1089/ten.TEA.2018.0147. [PUBMED]
  55. Fayomi AP, Orwig KE. Spermatogonial stem cells and spermatogenesis in mice, monkeys and men. Stem Cell Res. 2018. 29:207-214. [PUBMED]
  56. Fang F, Angulo B, Xia N, Sukhwani M, Wang Z, Carey CC, Mazurie A, Cui J, Wilkinson R, Wiedenheft B, Irie N, Surani MA, Orwig KE, Reijo Pera RA. A PAX5-OCT4-PRDM1 developmental switch specifies human primordial germ cells. Nat Cell Biol. 2018. 20(6):655-665. [PUBMED]
  57. Garbuzov A, Pech MF, Hasegawa K, Sukhwani M, Zhang RJ, Orwig KE, Artandi SE. Purification of GFRa1+ and GFRa1- Spermatogonial Stem Cells Reveals a Niche Dependent Mechanism for Fate Determination. Stem Cell Reports. 2018. 10: 553-567. [PUBMED]
  58. Jakus AE, Laronda MM, Rashedi AS, Robinson CM, Lee C, Jordan SW, Orwig KE, Woodruff TK, Shah RN. Tissue Papers from Organ-Specific Decellularized Extracellular Matrices. Advanced Functional Materials 2017; 27:1-4. [PUBMED]
  59. Sosa E, Kim R, Rojas EJ, Hosohama L, Hennebold JD, Orwig KE, Clark AT. An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC90) from embryonic fibroblasts. 2017. Stem Cell Res. 21: 5-8. [PUBMED]
  60. Clark AT, Gkountela S, Chen D, Liu W, Sosa E, Sukhwani M, Hennebold JD, Orwig KE. Primate Primordial Germ Cells Acquire Transplantation Potential by Carnegie Stage 23. 2017. Stem Cell Reports. 1:329-341. [PUBMED]
  61. Singh D, Paduch DA, Schlegel PN, Orwig KE, Mielnik A, Bolyakov A, Wright WW. The production of glial cell line-derived neurotrophic factor by human sertoli cells is substantially reduced in sertoli cell-only testes. 2017. Hum Reprod. 5: 1108-1117. [PUBMED]
  62. Gassei K, Sheng Y, Fayomi A, Mital P, Sukhwani M, Lin CC, Peters KA, Althouse A, Valli H, Orwig KE. DDX4-EGFP transgenic rat model for the study of germline development and spermatogenesis. 2017. Biol Reprod. 3: 707-719. [PUBMED]
  63. Johnson EK, Finlayson C, Rowell EE, Gosiengfiao Y, Pavone ME, Lockart B, Orwig KE, Brannigan RE, Woodruff TK. Fertility Preservation for Pediatric Patients: Current State and Future Possibilities. 2017. J Urol. 17: 30223-9. [PUBMED]
  64. Sosa E, Kim R, Rojas EJ, Hosohama L, Hennebold JD, Orwig KE, Clark AT. An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC89) from embryonic fibroblasts. 2016. Stem Cell Res. 2:444-447. [PUBMED]
  65. Panula S, Reda A, Stukenborg JB, Ramathal C, Sukhwani M, Albalushi H, Edsgärd D, Nakamura M, Söder O, Orwig KE, Yamanaka S, Reijo Pera RA, Hovatta O. Over Expression of NANOS3 and DAZL in Human Embryonic Stem Cells. 2016. PLos One. 11(10):e0165268. [PUBMED]
  66. Song HW, Bettegowda A, Lake BB, Zhao AH, Skarbrevik D, Babajanian E, Sukhwani M, Shum EY, Phan MH, Plank TD, Richardson ME, Ramaiah M, Sridhar V, de Rooij DG, Orwig KE, Zhang K, Wilkinson MF. The Homeobox Transcription Factor RHOX10 Drives Mouse Spermatogonial Stem Cell Establishment. 2016. Cell Reports. 1:149-64. [PUBMED]
  67. Simerly C, Castro C, Hartnett C, Lin CC, Sukhwani M, Orwig K, Schatten G. Post-Testicular Sperm Maturation: Centriole Pairs, Found in Upper Epididymis, are Destroyed Prior to Sperm’s Release at Ejaculation. 2016. Sci Rep. 6:31816. [PUBMED]
  68. Gassei K, Orwig KE.  Experimental methods to preserve male fertility and treat male factor infertility. 2016. Fertil Steril. 2:556-566. [PUBMED]
  69. Toocheck C, Clister T, Shupe J, Crum C, Ravindranathan P, Lee TK, Ahn JM, Raj GV, Sukhwani M, Orwig KE, Walker WH. Mouse spermatogenesis requires classical and nonclassical testosterone signaling. 2016. Biol Reprod. 1:11. [PUBMED]
  70. Pech MF, Garbuzov A, Hasegawa K, Sukhwani M, Zhang RJ, Benayoun BA,  Brockman SA, Lin S, Brunet A, Orwig KE, Artandi SE. High telomerase is a hallmark of undifferentiated spermatogonia and is required for maintenance of male germline stem cells. 2015. Genes Dev. 23:2420-2434. [PUBMED]
  71. Ramathal C, Angulo B, Sukhwani M, Cui J, Durruthy-Durruthy J,  Fang F, Schanes P, Turek PJ, Orwig KE, Reijo Pera RA. DDX3Y gene rescue of a Y chromosome AZFa deletion restores germ cell formation and transcriptional programs. 2015. Sci Rep. 5:15041. [PUBMED]
  72. Dominguez AA, Chiang HR, Sukhwani M, Orwig KE, Reijo Pera RA. Human germ cell formation in xenotransplants of induced pluripotent stem cells carrying X chromosome aneuploidies. 2014. Sci Rep. 4:6432. [PUBMED]
  73. Gassei K, Valli H, Orwig KE. Whole-mount immunohistochemistry to study spermatogonial stem cells and spermatogenic lineage development in mice, monkeys, and humans. 2014. Methods Mol Biol. 1210:193-202. [PUBMED]
  74. Medrano JV, Martínez-Arroyo AM, Sukhwani M, Noguera I,  Quiñonero A, Martínez-Jabaloyas JM, Pellicer A, Remohí J, Orwig KE, Simón C. 2014. Germ cell transplantation into mouse testes procedure.  2014. Fertil Steril. 102(4):e11-2. [PUBMED]
  75. Valli H, Sukhwani M, Dovey SL, Peters KA, Donohue J, Castro CA, Chu T, Marshall GR, Orwig KE. Fluorescence- and magnetic-activated cell sorting strategies to isolate and enrich human spermatogonial stem cells. 2014. Fertil Steril. 102(2):566-580. [PUBMED]
  76. Ramathal C, Durruthy Durruthy J, Sukhwani M, Arakaki JE, Turek PJ, Orwig KE, Reijo Pera RA. Fate of iPSCs derived from azoospermic and fertile men following xenotransplantation to murine seminiferous tubules. 2014. Cell Rep. 7(4):1284-97. [PUBMED]
  77. Durruthy Durruthy J, Ramathal C, Sukhwani M, Arakaki JE, Turek PJ, Orwig KE, Reijo Pera RA. Fate of induced pluripotent stem cells following transplantation to murine seminiferous tubules. 2014. Hum Mol Genet. 23(12):3071-84. [PUBMED]
  78. Valli H, Phillips BT, Shetty G, Byrne JA, Clark AT, Meistrich ML, Orwig KE. Germline stem cells: toward the regeneration of spermatogenesis. 2014. Fertil Steril. 101(1):3-13. [PUBMED]
  79. Zhang Z, Zhang S, Huang X, Orwig KE, Sheng Y. 2013. Rapid Assembly of Customized TALENs into Multiple Delivery Systems. PLoS One. 8(11):e80281. [PUBMED]
  80. Shetty G, Uthamanthil RK, Zhou W, Shao SH, Weng CC, Tailor RC, Herman BP, Orwig KE, Meistrich ML. 2013. Hormone suppression with GnRH antagonist promotes spermatogenic recovery from transplanted spermatogonial stem cells in irradiated cynomolgus monkeys. Andrology. 1(6):886-98. [PUBMED]
  81. Dovey SL, Valli H, Hermann BP, Sukhwani M, Donohue J, Castro CA, Chu T, Sanfilippo JS, Orwig KE. 2013. Eliminating malignant contamination from therapeutic human spermatogonial stem cells. J Clin Invest. 123(4):1833-43. [PUBMED]
  82. Skaznik-Wikiel ME, McGuire MM, Sukhwani M, Donohue J, Chu T, Krivak TC, Rajkovic A, Orwig KE. 2013. Granulocyte colony-stimulating factor with or without stem cell factor extends time to premature ovarian insufficiency in female mice treated with alkylating chemotherapy. Fertil Steril. 99(7):2045-54. [PUBMED]
  83. Gassei K and Orwig KE. 2013. Sall4 expression in gonocytes and spermatogonial clones of postnatal mouse testes. PLoS One. 8(1):e53976. [PUBMED]
  84. Hermann BP, Sukhwani M, Winkler F, Pascarella JN, Peters KA, Sheng Y, Valli H, Rodriguez M, Ezzelarab M, Dargo G, Peterson K, Masterson K, Ramsey C, Ward T, Lienesch M, Volk A, Cooper DK, Thomson AW, Kiss JE, Penedo MC, Schatten GP, Mitalipov S, Orwig KE. 2012. Spermatogonial stem cell transplantation into rhesus testes regenerates spermatogenesis producing functional sperm. Cell Stem Cell. 11(5):715-26. [PUBMED]
  85. Easley CA 4th, Phillips BT, McGuire MM, Barringer JM, Valli H, Hermann BP, Simerly CR, Rajkovic A, Miki T, Orwig KE, Schatten GP. Direct differentiation of human pluripotent stem cells into haploid spermatogenic cells. 2012. Cell Rep. 2(3):440-6. [PUBMED]
  86. Suzuki H, Ahn HW, Chu T, Bowden W, Gassei K, Orwig K, Rajkovic A. 2012. SOHLH1 and SOHLH2 coordinate spermatogonial differentiation. Dev Biol. 361(2):301-12. [PUBMED]
  87. Clark AT, Phillips BT, Orwig KE. 2011. Fruitful progress to fertility: male fertility in the test tube. Nat Med. 17(12):1564-5. [PUBMED]
  88. Hermann BP, Sukhwani M, Salati J, Sheng Y, Chu T, Orwig KE. 2011. Separating spermatogonia from cancer cells in contaminated prepubertal primate testis cell suspensions. Hum Reprod. 26(12):3222-31. [PUBMED]
  89. Hermann BP, Phillips BT, Orwig KE. 2011. The elusive spermatogonial stem cell marker? Biol Reprod. Aug;85(2):221-3. [PUBMED]
  90. Honaramooz A, Schlatt S, Orwig K, Kim NH. Recent advances in reproductive technologies. 2011. Vet Med Int. 915031. Epub 2011 Jun 22 .[PUBMED]
  91. Simerly C, McFarland D, Castro C, Lin CC, Redinger C, Jacoby E, Mich-Basso J, Orwig K, Mills P, Ahrens E,  Navara C, Schatten G. 2011. Interspecies chimera between primate embryonic stem cells and mouse embryos: monkey ESCs engraft into mouse embryos, but not post-implantation fetuses. Stem Cell Res. 7(1):28-40. [PUBMED]
  92. Phillips BT, Gassei K , Orwig KE. 2010. Spermatogonial stem cell regulation and spermatogenesis. Phil Trans R Soc B ; 365, 1663-1678. [PUBMED]
  93. Ben-Yehudah A, Easley 4th CA, Hermann BP, Castro C, Simerly C, Orwig KE, Mitalipov S, Schatten G. 2010. Systems biology discoveries using non-human primate pluripotent stem and germ cells: novel gene and genomic imprinting interactions as well as unique expression patterns.Stem Cell Res Ther. 1(3):24. [PUBMED]
  94. Hermann BP, Sukhwani M, Hansel MC, Orwig KE. 2010. Spermatogonial stem cells in higher primates: are there differences from those in rodents? Reproduction. 139: 479–493; DOI: 10.1530/REP-09-0255 [PUBMED]
  95. Sheng Y, Lin CC, Yue J, Sukhwani M, Shuttleworth JJ, Chu T, Orwig KE. Generation and characterization of a Tet-On (rtTA-M2) transgenic rat. 2010. BMC Developmental Biology. 10:17. [PUBMED]
  96. Hermann BP, Sukhwani M, Simorangkir DR, Chu T, Plant TM, Orwig KE. 2009. Molecular Dissection of the Male Germ Cell Lineage Identifies Putative Spermatoganial Stem Cells in Rhesus Macaques. Hum Reprod. 24(7): 1704-16. [PUBMED]
  97. Escamilla-Hernandez R, Little-Ihrig L , Orwig KE , Yue J, Chandran U, Zeleznik AJ. 2008. Constitutively Active Protein Kinase A Qualitatively Mimics the Effects of Follicle-Stimulating Hormone on Granulosa Cell Differentiation. Mol Endocrinol. 22(8):1842-52. [PUBMED]
  98. Orwig KE, Ryu BY, Master S, Phillips BT, Mack M, Avarbock MR, Brinster RL. 2008. Genes Involved in Post-transcriptional Regulation are Over Represented in Stem/progenitor Spermatogonia of Cryptorchid Mouse Testes. Stem Cells. 26(4):927-38. [PUBMED]
  99. Yue J, Sheng Y, Orwig KE. 2008. Identification of Novel Homologous microRNA Genes in the Rhesus Macaque Genome. BMC Genomics. 9(1): 8. [PUBMED]
  100. Hermann BP, Sukhwani M, Lin CC, Sheng Y, Tomko J, Rodriguez M, Shuttleworth JJ, McFarland D, Hobbs RM, Pandolfi PP, Schatten GP, Orwig KE. 2007. Characterization, Cryopreservation and Ablation of Spermatogonial Stem Cells In Adult Rhesus Macaques. Stem Cells. 25(9):2330-2338. [PUBMED]
  101. Navara CS, Mich-Basso JD, Redinger CJ, Ben-Yehudah A, Jacoby E, Kovkarova-Naumovski E, Sukhwani M, Orwig K, Kaminski N, Castro CA, Simerly CR, Schatten G. 2007. Pedigreed Primate Embryonic Stem Cells, Express Homogeneous Familial Gene Profiles. Stem Cells. 25(11):2695-704. [PUBMED]
  102. Ryu BY, Orwig KE,  Oatley JM, Lin CC, Chang LJ, Avarbock MR, Brinster RL. 2007. Efficient Generation of Transgenic Rats Through the Male Germline using Lentiviral Transduction and Transplantation of Spermatogonial Stem Cells. J Androl. 28(2):353-60. [PUBMED]
  103. Ryu BY, Orwig KE, Oatley JM, Avarbock MR, Brinster RL. 2006. Effects of Aging and Niche Microenvironment on Spermatogonial Stem Cell Self-Renewal.  Stem Cells. 24(6):1505-1511. [PUBMED]
  104. Buageaw A, Sukhwani M, Ben-Yehudah A, Ehmcke J, Rawe VY,  Pholpramool C, Orwig KE, Schlatt S. 2005. GDNF Family Receptor alpha1 Phenotype of Spermatogonial Stem Cells in Immature Mouse Testes. Biol. Reprod. 73(5):1011-1016. [PUBMED]
  105. Orwig KE, Schlatt S. 2005. Cryopreservation and transplantation of spermatogonia and testicular tissue for preservation of male fertility.J Natl Cancer Inst Monogr. 34:51-56. [PUBMED]
  106. Ryu BY, Orwig KE, Kubota H, Avarbock MR, Brinster RL. 2004. Phenotypic and functional characteristics of spermatogonial stem cells in rats. Dev. Biol. 274:158-170. [PUBMED]
  107. Costoya JA, Hobbs RM, Barna M, Cattoretti G, Manova K, Sukhwani M, Orwig KE, Wolgemuth DJ, Pandolfi PP. 2004. Essential role of Plzf in maintenance of spermatogonial stem cells. Nature Genetics. 36: 653-659. [PUBMED]
  108. B.Y. Ryu, K.E. Orwig, M.R. Avarbock, R.L. Brinster. 2003. Stem cell and niche development in the postnatal rat testis. Dev. Biol. 263:253-263. [PUBMED]
  109. T. Shinohara, K.E. Orwig, M.R. Avarbock, R.L. Brinster. 2003. Restoration of spermatogenesis in infertile mice by Sertoli cell transplantation. Biol. Reprod. 68:1064-1071. [PUBMED]
  110. C.J. Brinster, B.-Y. Ryu, L. Karagenc, R.L. Brinster, K.E. Orwig. 2003. Restoration of fertility by germ cell transplantation requires effective recipient preparation. Biol. Reprod. 69:412-420. [PUBMED]
  111. K.E. Orwig, B.-Y. Ryu, M.R. Avarbock, R.L. Brinster. 2002. Male germ-line stem cell potential is predicted by morphology of cells in neonatal rat testes. Proc. Nat. Acad. Sci. USA. 99:11706-11711. [PUBMED]
  112. K.E. Orwig, M.R. Avarbock, R.L. Brinster. 2002. Retrovirus-mediated modification of male germline stem cells in rats. Biol. Reprod. 67:874-879. [PUBMED]
  113. T. Shinohara, K.E. Orwig, M.R. Avarbock, R.L. Brinster. 2002. Germ line stem cell competition in postnatal mouse testes. Biol. Reprod. 66:1491-1497. [PUBMED]
  114. K.E. Orwig, T. Shinohara, M.R. Avarbock, R.L. Brinster. 2002. Functional analysis of stem cells in the adult rat testis. Biol. Reprod. 66:944-949. [PUBMED]
  115. M. Nagano, C.J. Brinster, K.E. Orwig, B.-Y. Ryu, M.R. Avarbock, R.L. Brinster. 2001. Transgenic mice produced by retroviral transduction of male germ-line stem cells. Proc. Nat. Acad. Sci. USA. 98:13090-13095. [PUBMED]
  116. T. Shinohara, K.E. Orwig, M.R. Avarbock, R.L. Brinster. 2001. Remodeling of the postnatal mouse testis is accompanied by dramatic changes in stem cell number and niche accessibility. Proc. Nat. Acad. Sci. USA. 98:6186-6191. [PUBMED]
  117. T. Shinohara, K.E. Orwig, M.R. Avarbock, R.L. Brinster. 2000. Spermatogonial stem cell enrichment by multiparameter selection of mouse testis cells. Proc. Nat. Acad. Sci. USA. 97:8346-8351. [PUBMED]
  118. S. Filley, S. Supansic, U. Salli, K. Orwig, F. Stormshak. 2000. Myristoylated alanine-rich c kinase substrate protein and mRNA in bovine corpus luteum during the estrous cycle. Endocrine. 12:289-294. [PUBMED]
  119. K.E. Orwig and M.J. Soares. 1999. Transcriptional activation of the decidual/trophoblast prolactin-related protein gene. Endocrinology. 140:4032-4039. [PUBMED]
  120. G.D. Dai, B.M. Chapman, B. Liu, K.E. Orwig, D. Wang, R.A. White, B. Preuett, M.M. Soares. 1998. A new member of the mouse prolactin (PRL)-like protein-C subfamily, PRL-like protein-Ca: Structure and Expression. Endocrinology. 139:5157-5163. [PUBMED]
  121. K.E. Orwig, M.W. Wolfe, C.B. Cohick, G. Dai, T.J. Peters, M.J. Soares. 1998. Trophoblast-specific regulation of endocrine-related genes. Trophoblast Research. 12:65-85.
  122. H. Müller, K.E. Orwig, M.J. Soares. 1998. Identification of two new members of the mouse prolactin gene family. Biochim. Biophys. Acta 1396:251-258. [PUBMED]
  123. M.J. Soares, H. Müller, K.E. Orwig, T.J. Peters, G. Dai. 1998. The Uteroplacental prolactin family and pregnancy. Biol. Reprod. 58:273-284. [PUBMED]
  124. H. Müller, R. Ishimura, K.E. Orwig, B. Liu, M.J. Soares. 1998. Homologues for prolactin-like protein-A and B are present in the mouse. Biol. Reprod. 58:45-51. [PUBMED]
  125. K.E. Orwig, R. Ishimura, H. Müller, B. Liu and M.J. Soares. 1997. Identification and characterization of a mouse homolog for decidual/trophoblast prolactin-related protein. Endocrinology. 138:5511-5517. [PUBMED]
  126. K.E. Orwig, C.A. Rasmussen and M.J. Soares. 1997. Decidual Signals in the establishment of pregnancy: the prolactin family. Trophoblast Research. 10:329-343.
  127. K.E. Orwig, G. Dai, C.A. Rasmussen and M.J. Soares. 1997. Decidual/trophoblast prolactin-related protein: characterization of gene structure and cell-specific expression. Endocrinology. 138:2491-2500. [PUBMED]
  128. C.A. Rasmussen, K.E. Orwig, S. Vellucci and M.J. Soares. 1997. Dual expression of prolactin-related protein in decidua and trophoblast tissues during pregnancy. Biol. Reprod. 56:647-654. [PUBMED]
  129. C.A. Rasmussen, K. Hashizume, K.E. Orwig, L. Xu and M.J. Soares. 1996. Decidual prolactin-related protein: heterologous expression and characterization. Endocrinology. 137:5558-5566. [PUBMED]
  130. M.J. Soares, B.M. Chapman, C.B. Cohick, C.A. Rasmussen, G. Dai, T. Kamei and K.E. Orwig. 1996. Differentiation of trophoblast endocrine cells. Placenta. 17:277-289. [PUBMED]
  131. F. Stormshak, K.E. Orwig and J.E. Bertrand. 1995. Dynamics of molecular mechanisms underlying ovarian oxytocin secretion. Journal of Reproduction and Fertility. 49:379-390. [PUBMED]
  132. K.E. Orwig, J.E. Bertrand, B.-R.Ou, N.E. Forsberg and F. Stormshak. 1994. Immunochemical characterization and cellular distribution of protein kinase C isozymes in the bovine corpus luteum. Comparative Biochemistry and Physiology. 108B:53-57. [PUBMED]
  133. K.E. Orwig, J.E. Bertrand, B.-R.Ou, N.E. Forsberg and F.Stormshak. 1994. Involvement of protein kinase C, calpains and calpastatin in prostaglandin F2*-induced oxytocin secretion from the bovine corpus luteum. Endocrinology. 134:78-83. [PUBMED]
  134. K.E. Orwig, S. Leers-Sucheta, M.F. Moghaddam, Z.D. Jiang, W.R. Gerwick and F. Stormshak. 1992. Unique metabolites of eicosapentaenoic acid interfere with corpus luteum function in the ewe. Prostaglandins. 44:519-530. [PUBMED]

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